Bovine serum albumin-bovine hemoglobin conjugate as a candidate blood substitute |
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Authors: | Tao Hu Zhiguo Su |
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Institution: | (1) National Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100080, P.O. Box 353, P.R. China |
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Abstract: | A bovine serum albumin-bovine hemoglobin conjugate was prepared using 3-maleimidobenzoic acid N-hydroxysuccinimide ester as cross-linker. The conjugate was purified using DEAE Sepharose. It had an M
r of 127 kDa. Its P50 (half-saturated O2 pressure) value and Hill coefficient were 27 mm Hg and 2, respectively. |
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Keywords: | blood substitute bovine hemoglobin bovine serum albumin 3-maleimidobenzoic acid N-hydroxysuccinimide ester |
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