Quantitative measurement of SV40 T-antigen production |
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| Authors: | M Horan P K Horan C A Williams |
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| Institution: | 1. Los Alamos Scientific Laboratory, University of California, Los Alamos, New Mex. 87544 USA;2. State University of New York, Purchase, N.Y. 10577, USA |
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| Abstract: | African green monkey cells (CV-1) were infected with SV40 virus at high multiplicities of infection (MOI), and the production of T-antigen was studied. A new instrumentation, flow microfluorometry, coupled with indirect immunofluorescence permitted quantitative evaluation of this antigen. Optimum conditions were determined for antibody excess. Antigen production was not detected for the first 6 h post-infection. The value of this technology is discussed in relation to quantitative evaluation of expression of cellular antigens. |
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