扎龙芦苇遗传多样性ISSR分析

采用ISSR分子标记技术对扎龙湿地的5个野生芦苇居群进行了遗传多样性研究.从75条ISSR引物中筛选出10个特异性强、稳定性好的引物进行ISSR分析.共获得96个位点,其中多态位点94个,多态位点百分率为97.92％.PopGene分析结果表明:居群的平均多态位点百分率为56.46％,Shannon's多样性指数(J)...

Genetic diversity of Phragmites australis populations from Zhalong based on ISSR analysis

The genetic diversity of 5 wild Phragmites australis populations from Zhalong was estimated using inter-simple sequence repeat(ISSR)markers,using ten specific and stable primers selected from 75 primers.A total of 96 sites were generated,94 of which(97.92%)were polymorphic.The data was analyzed by PopGene.The average percentage of polymorphicloci of populations was 56.46% and demonstrated high genetic diversity(the average value of Shannon's index(I)was 0.4783).To some extent,both gene flow and genetic diversity were present among populations(Nm was 0.9249,average Nei's index was 0.3150,and average Gst was 0.3509).The gene proportion of diversity reached 64.91% within population but was 35.09% among populations.This demonstrated that genetic variation of P.australis populations was mainly within populations.According to cluster analysis,the populations were classified into two large groups:the populations(K,T and J)grouped together,whereas populations(M and Z)grouped together.These results would supplement the information for protection and utilization of P.australis and also provide further data for the study of genetic variation and species differentiation of P.australis.