| 巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究 |
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| 引用本文： | 韩素菊,黎云祥.巫山淫羊藿愈伤组织细胞的悬浮培养条件优化的初步研究[J].广西植物,2011,31(6):827-831. |
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| 作者姓名： | 韩素菊 黎云祥 |
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| 作者单位： | 1. 绵阳师范学院,四川绵阳,621000
2. 西华师范大学,西南野生动植物资源保护教育部重点实验室,四川南充637002 |
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| 基金项目： | 四川省教育厅重点项目，四川省科技厅应用基础研究项目，四川省重点实验室开放基金 |
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| 摘 要： | 通过研究接种量、激素配比、糖浓度、培养基种类对巫山淫羊藿悬浮培养细胞生长及其愈伤组织黄酮类含量的影响,建立了巫山淫羊藿细胞悬浮培养的技术体系.结果表明:巫山淫羊藿愈伤组织细胞悬浮培养在B5基本培养基中并附加1.0 mg·L-12,4-D和0.2 mg· L-1BA,蔗糖浓度40 g·L-1,接种量每30 mL为鲜重2 ...
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| 关 键 词： | 巫山淫羊藿 细胞悬浮培养 黄酮类化合物 |
Primary study on suspension cell culture of Epimediun wushanense |
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| HAN Su-Ju,LI Yun-Xiang.Primary study on suspension cell culture of Epimediun wushanense[J].Guihaia,2011,31(6):827-831. |
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| Authors: | HAN Su-Ju LI Yun-Xiang |
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| Abstract: | The technological system of cell suspension culture by the flavonoids content of E.wushanense was established through studing the effect of inoculation concentration,hormone combination,sugar concentration and various medias.The result showed that the suspension culture optimum medium of E.wushanense was B5 medium supplemented with 1.0 mg·L-12,4-D and 0.2 mg·L-16-BA,the sugar concentration was 4.0% and the optimum inoculation concentration was 2 g per 30 mL.The highest content of the total flavonoids appeared on the medium. |
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| Keywords: | Epimediun wushanense suspension cell culture flavonoids |
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