The effect of synthetic glucocorticoid, dexamethasone on CYP1A1 inducibility in adult rat and human hepatocytes |
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| Authors: | Monostory Katalin Kohalmy Krisztina Prough Russell A Kóbori László Vereczkey László |
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| Affiliation: | Chemical Research Center, Hungarian Academy of Sciences, PO Box 17, H-1525 Budapest, Hungary. monostor@chemres.hu |
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| Abstract: | Glucocorticoids act synergistically with polycyclic aromatic hydrocarbons in increasing mRNA and protein levels of CYP1A1 in rat liver. The action of dexamethasone to modify CYP1A1 expression has been investigated in adult human hepatocytes. The effect of dexamethasone on the induction of CYP1A1 by 3-methylcholanthrene is different in rat and human liver cells. Dexamethasone potentiates the induction of CYP1A1 about 3- to 4-fold in rat cells. In human hepatocytes, it reduces CYP1A1 induction by 50-60% at enzyme protein level, while it does not have an effect on CYP1A1 mRNA amount. |
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| Keywords: | AhR, arylhydrocarbon receptor GR, glucocorticoid receptor GRE, glucocorticoid responsive element HBSS, Hank’s balanced salt solution PAH, polycyclic aromatic hydrocarbon P450, cytochrome P450 |
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