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Complete type III secretion system of a mesophilic Aeromonas hydrophila strain
Authors:Vilches Silvia  Urgell Cecilia  Merino Susana  Chacón Matilde R  Soler Lara  Castro-Escarpulli Graciela  Figueras Maria Jose  Tomás Juan M
Institution:Departamento Microbiología, Facultad Biología, Universidad de Barcelona, Barcelona, Spain.
Abstract:We have investigated the existence and genetic organization of a functional type III secretion system (TTSS) in a mesophilic Aeromonas strain by initially using the Aeromonas hydrophila strain AH-3. We report for the first time the complete TTSS DNA sequence of an Aeromonas strain that comprises 35 genes organized in a similar disposition as that in Pseudomonas aeruginosa. Using several gene probes, we also determined the presence of a TTSS in clinical or environmental strains of different Aeromonas species: A. hydrophila, A. veronii, and A. caviae. By using one of the TTSS genes (ascV), we were able to obtain a defined insertion mutant in strain AH-3 (AH-3AscV), which showed reduced toxicity and virulence in comparison with the wild-type strain. Complementation of the mutant strain with a plasmid vector carrying ascV was fully able to restore the wild-type toxicity and virulence.
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