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Identification of gibberellin A1 in the embryo suspensor of Phaseolus coccineus
Authors:A Alpi  R Lorenzi  PG Cionini  A Bennici  F D'Amato
Institution:(1) Istituto di Orticoltura e Floricoltura, Pisa, Italy;(2) Istituto di Genetica della Università, Pisa, Italy;(3) Istituto di Genetica della Università, Via Matteotti I/A, I-56100 Pisa, Italy
Abstract:By combined gas chromatography-mass spectrometry the gibberellin present in suspensors of heart-shaped embryos of Phaseolus coccineus has been identified as Gibberellin A1 (GA1). The amount of GA1 in 2000 suspensors (452 mg), as estimated by gas chromatography. was 4mgrg. The presence of GA1 in suspensors of P. coccineus is discussed in relation to our present knowledge of the occurrence of many gibberellins in developing seeds and immature fruits of the same species.Abbreviations FID flame ionization detector - GA gibberellin - GC gas chromatography - MS mass spectrometry - PGC preparative gas chromatography - Stage A heart-shaped embryo - Stage B cotytedonary embryo - TMS trimethylsilyl
Keywords:Embryo suspensor  Gibberellin  Phaseolus
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