美浮特皮肤抗菌液对致足癣真菌抗真菌后效应的体外研究

目的：评价美浮特^R皮肤抗菌液对致足癣真菌皮肤癣菌和白念珠菌的体外抗真菌活性及抗真菌后效应。方法采用美国 CLSI M27-A3和 M38-A2方案测定美浮特皮肤抗菌液对足癣常见致病真菌的最低抑菌浓度（ MIC）；并以白念珠菌（ATCC90028）为指示菌测定美浮特皮肤抗菌液测定时间-杀菌曲线，同时测定其对白念珠菌的抗真菌后效应（post-antifungal effect，PAFE）。结果美浮特皮肤抗菌液对4属6种57株 MIC 的范围为1：40-1：160、MIC50为1：80、MIC90为1：40；对白念珠菌的 MIC 范围为1：40-1：80、对皮肤癣菌的 MIC 范围为1：40-1：160。该抗菌液具有很强的杀菌作用，且随着药物浓度的降低，杀菌速度和程度随之变化。该抗菌液对白念珠菌0.5MIC、MIC、2MIC 的 PAFE 分别为0.85 h、2.1 h、3.59 h；且 PAFE 时间的延长与药物浓度呈正相关。结论美浮特^R皮肤抗菌液对致病真菌皮肤癣菌、白念珠菌具有快速、有效、持续的杀菌作用，该抗菌液对皮肤癣菌较白念珠菌具有更强的抗真菌作用。且该抗菌液对白念珠菌具有较长时间的后效应，可以广泛应用于临床治疗皮肤癣菌及白念珠菌所致的感染。

Post-antifungal effect of Meifute antifungal solution against fungal induced tinea pedis in vitro

Objective To investigate antifugal activity and the post-antifungal effect（PAFE）of Meifute antifungal solution a-gainst fungal induced tinea pedis. Methods We detected the minimum inhibitory concentration（MIC）of Meifute antifungal solu-tion against fungal common induced tinea pedis using the United States CLSI M27-A3 and M38-2 scheme. Time-killing curve and post antifungal effects of Meifute antifungal solution against Candida albicans（ATCC90028）as the indicator fungal were measured. Result The MIC range,MIC50 and MIC90 of Meifute antifungal solution against 57 species were 1： 40 - 1： 160,1： 80 and 1： 40 respectively. The ranges of the solution against Candida albicans and dermatophytes were 1： 40 - 1： 80 and 1： 40 - 1： 160. This antifungal solution has a strong antifungal effect. The degree and speed of the killing fungal were changed with the decrease of drug concentration. The PAFE of 0. 5MIC,1MIC and 2MIC were 0. 85 h,2. 1 h and 3. 59 h respectively,and the prolonged of PAFE was positively correlated to the antifungal solution concentration. Conclusion Meifute antifungal solution has a fast,sustained effect a-gainst Candida albicans and dermatophtes. Comparing with the effect on Candida albicans ,the anigfungal solution have stronger antifungal effect on dermatophtes. The antifunal solution also have a long period of PAFE. These results showed that Meifute antifun-gal solution can be widely used to treat the superficial fungal infection of dermatophytes and Candida albicans .