| 肌糖原磷酸化酶a,b亚基的杂交 |
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| 引用本文： | 唐国庆,吴田平.肌糖原磷酸化酶a,b亚基的杂交[J].生物化学与生物物理学报,1995,27(2):117-121. |
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| 作者姓名： | 唐国庆 吴田平 |
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| 作者单位： | 中国科学院上海生物化学研究所 |
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| 摘 要： | 本文用标记在肌糖原磷酸化酶ａ（ＧＰＡ）亚基上的荧光探针ＩＡＥＤＡＮＳ与标记在肌糖原磷酸化酶ｂ（ＧＰＢ）亚基上的ＩＡＦ之间无辐射能量转移；高压力下亚基交换动力学和外源荧光探针荧光偏振等三种方法证实了肌糖原磷酸化酶ａ、ｂ的亚基可相互交换，形成磷酸化酶ａ、ｂ的杂交形式。
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| 关 键 词： | 肌糖原磷酸化酶 杂交 能量转移 荧光偏振 亚基 |
Hybridization of the Subunits of Muscle Glycogen Phosphorylase A and B |
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| TANG Guo-Qing,WU Tian-Ping and RUAN Kang-Cheng.Hybridization of the Subunits of Muscle Glycogen Phosphorylase A and B[J].Acta Biochimica et Biophysica Sinica,1995,27(2):117-121. |
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| Authors: | TANG Guo-Qing WU Tian-Ping and RUAN Kang-Cheng |
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| Abstract: | Three methods,non-radiation energy transfer between IAEDANS labeled on muscle glycogen phosphorylase A(GPA) subunit and IAF labeled on muscle glycogen phosphorylase B(GPB) subunit,kinetics of subunit exchange under high pressure and measurement of the fluorescence polarization of the extrinsic fluorescent probe were used in this study.The results indicate that the GPA and GRB subunits can exchange to form hybrid of GPA and GPB. |
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| Keywords: | Muscle glycogen phosphorylase A and B Hybridization Energy transfer Fluorescence polarization |
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