Plants obtained from the Khair tree (Acacia catechu Willd.) using mature nodal segments |
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Authors: | K. Kaur B. Verma U. Kant |
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Affiliation: | (1) Plant Pathology and Tissue Culture Biotechnology Laboratory, Department of Botany, University of Rajasthan, Jaipur-302004, India, IN |
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Abstract: | An in vitro method for obtaining plants of Acacia catechu has been developed using nodal explants from mature `elite' trees growing in the field. Maximum shoot bud development (eight to ten) from a single explant was achieved on Murashige and Skoog (MS) medium supplemented with 6-benzylaminopurine (BAP) (4.0 mg/l) and α-naphthaleneacetic acid (0.5 mg/l). Addition of adenine sulphate (25.0 mg/l), ascorbic acid (20.0 mg/l) and glutamine (150.0 mg/l) to the medium was found beneficial for maximum shoot bud induction. The shoot buds developed into healthy and sturdy shoots on MS medium containing BAP and kinetin at 1.0 mg/l. Excised shoots were rooted on 1/4-strength MS medium with indole-3-acetic acid at 3.0 mg/l and 1.5% sucrose to obtain complete plants. Received: 17 June 1997 / Revision received: 11 September 1997 / Accepted: 27 September 1997 |
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Keywords: | Acacia catechu Clonal propagation Mature nodal explants |
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