Identification of Basic Nuclear Proteins by their Boronate Complex |
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| Authors: | K. Jobst . Lakatos A. Horv th |
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| Affiliation: | Department of Clinical Chemistry, University School of Medicine, Pécs, Hungary. |
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| Abstract: | A new histochemical reaction for the identification of histone type basic proteins has been developed. Carbonyldiimidazol is used to activate the basic proteins of TCA-extracted nuclei, their m-aminophenylboronic acid complex is prepared, and the DNA-free, histone-containing nuclei are stained with toluidine blue at pH 5.5. |
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| Keywords: | histone-blocking histone-CDI histone-boronate basic proteins toluidine blue |
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