PR-10 protein is induced by copper stress in roots and leaves of a Cu/Zn tolerant clone of birch, Betula pendula |
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| Authors: | M Utriainen H Kokko S Auriola O Sarrazin & S Kärenlampi |
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| Institution: | Department of Biochemistry and Biotechnology,,;Department of Pharmaceutical Chemistry, University of Kuopio, PO Box 1627, FIN-70211 Kuopio, Finland, and,;Department of Biochemistry, Universite Paris 7, place Jussieu, 75251 Paris Cedex 05, France |
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| Abstract: | The response of a Cu- and Zn-tolerant birch ( Betula pendula ) clone to copper stress was investigated. The plants were exposed to control and EC50 concentrations of Cu (0·3 and 30 μ M CuSO4, respectively) for 7 d in hydroponic culture. Total proteins were extracted from the roots and leaves and separated by two-dimensional polyacrylamide gel electrophoresis. The differences in protein patterns on silver- or Coomassie-stained gels were analysed. The most apparent quantitative difference was the increase in the amount of a 17 kDa polypeptide caused by Cu stress in both roots and leaves. The protein was identified as Bet v 1-Sc3 (according to the current nomenclature PR-10c) using N-terminal amino acid sequencing and on-line high-performance liquid chromatography/electrospray ionization/ion trap mass spectrometry. The present results indicate that PR-10 is not only activated by pathogens but also by excessive amounts of copper ions. PR-10 proteins in birch have been reported earlier not to be induced by Ag, Li or Cd in birch suspension culture, but Cu has not been previously tested. |
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| Keywords: | birch pollen allergen copper Cu/Zn resistance ESI-MS heavy metals pathogenesis-related protein two-dimensional electrophoresis |
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