| 钙离子对细胞分裂素延缓水稻叶片衰老的抑制作用 |
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| 引用本文： | 彭新湘 Yamau.,M.钙离子对细胞分裂素延缓水稻叶片衰老的抑制作用[J].Acta Botanica Sinica,1996,38(6):463-466. |
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| 作者姓名： | 彭新湘 Yamau. M |
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| 作者单位： | 华南农业大学!广州510642(彭新湘)，国际水稻研究所!菲律宾(YamauchiM) |
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| 基金项目： | Supported by the N tional Natural Science Foundation of China |
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| 摘 要： | 单独使用细胞分裂素 (BA和 Zeatin,1 0 -9～ 1 0 -5 mol/ L和 Ca2 (1 0 -3 mol/ L)处理水稻离体叶片时 ,二者均对叶片衰老有延缓作用。但当用 Ca2 和细胞分裂素同时处理叶片时 ,细胞分裂素延缓衰老的作用受到 Ca2 的明显抑制。进一步研究表明 ,细胞分裂素和 Ca2 并未协同刺激水稻离体叶片的乙烯生成 ,这样排除了通过乙烯促进叶片衰老的可能性。用可提高细胞质 Ca2 浓度的钙通道载体 A2 31 87处理叶片时 ,可延缓叶片衰老 ;而用可降低胞质 Ca2 浓度的试剂 ,如 EGTA、La Cl3 、Verapamil、氯丙嗪等(1 0 -3 mol/ L)处理叶片时 ,可促进叶片衰老 ,进而排除了细胞分裂素促进 Ca2 的吸收而加快衰老的可能性。
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| 关 键 词： | 细胞分裂素 Ca~(2 ) 衰老 水稻 |
EXOGENOUS Ca~(2 ) INHIBITS SENESCENCE-RETARDING EFFECT OF CYTOKININS IN DETACHED RICE LEAVES |
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| Authors: | Peng Xin xiang [WTBZ] |
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| Institution: | Peng Xin xiang [WT6BZ] |
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| Abstract: | Cytokinins and Ca 2 singly retarded senescence of detached rice leaves.When Ca 2 was applied together with cytokinins,the effectiveness of cytokinins was significantly reduced.Ca 2 and cytokinins did not stimulate ethylene production synergistically,ruling out the possibility that ethylene was involved in the inhibition of cytokinin induced senescence retarding effect by Ca 2 .The experiment with specific compounds known to increase (Ca ionophore A23187),or decrease (EGTA,LaCl 3,Verapamil,chlorpromazine) cytosolic Ca 2 level indicated that the elevated cytosolic Ca 2 retards senescence. |
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| Keywords: | Cytokinin Ca 2 Senescence Rice |
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