| 利用栝楼发根生产天花粉蛋白的研究 |
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| 引用本文： | 邱德有,朱至清.利用栝楼发根生产天花粉蛋白的研究[J].Acta Botanica Sinica,1996,38(6):439-443. |
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| 作者姓名： | 邱德有 朱至清 |
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| 作者单位： | 北京大学生命科学学院!北京100871(邱德有，朱微)，中国科学院植物研究所!北京100093(朱至清) |
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| 摘 要： | 利用发根农杆菌 (Agrobacterium rhizogenes)菌株 R1 60 1 ,成功地从药用植物栝楼 (Trichosantheskirilowii Maxim.)中诱导出发根并建立了栝楼发根的离体培养系统。通过蛋白质印迹法对栝楼发根的天花粉蛋白进行了鉴定。利用 SDS- PAGE和 TLC扫描 ,测定出每克鲜重栝楼发根中天花粉蛋白的含量可达 8.1 6mg。
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| 关 键 词： | 栝楼 发根 天花粉蛋白 |
PRODUCTION OF TRICHOSANTHIN FROM THE HAIRY ROOTS OF TRICHOSANTHES KIRILOWII MAXIM. |
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| Authors: | Qiu De you and Zhu Cheng [WTBZ] |
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| Institution: | Qiu De you and Zhu Cheng ** [WT6BZ] |
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| Abstract: | Hairy roots of Trichosanthes kirilowii Maxim. were successfully induced by infection with Agrobacterium rhizogenes strain R1601 and an in vitro culture system of the hairy roots was established.The presence of trichosanthin in the hairy roots of T. kirilowii was confirmed by western blotting.The yield of trichosanthin was determined to be 8.16 mg/g fr.wt with SDS PAGE gel electrophoresis and thin layer chromatography. |
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| Keywords: | Trichosanthes kirilowii Hairy roots Trichosanthin |
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