High-level expression of self-processed HIV-1 protease in Escherichia coli using a synthetic gene |
| |
Authors: | Z Hostomsky K Appelt R C Ogden |
| |
Affiliation: | Agouron Pharmaceuticals Inc., La Jolla, CA 92037. |
| |
Abstract: | A synthetic gene coding for HIV-1 protease (PR) has been constructed and a system for its efficient expression in E. coli has been established: PR is synthesized as a fusion protein with E. coli dihydrofolate reductase under the control of a bacteriophage T7 promoter. The synthetic gene was constructed to enable rapid construction of defined mutants by restriction fragment replacement. A set of mutants has been constructed which may facilitate elucidation of the mechanism of PR self-cleavage from polyprotein precursors. We have demonstrated that the C-terminal residue (Phe99 in the native sequence) of the processing intermediate is absolutely required for subsequent cleavage at the N-terminal cleavage site. The potential structural role of this residue is discussed with reference to the recently published HIV-1 PR structure. |
| |
Keywords: | |
|