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A Technique for Simultaneous Antikinetochore Immunofluorescence Staining and Q-Banding in Chromosomes from Human Lymphocytes
Authors:Lorraine M Cherry  Samir A Shah
Institution:  a Section of Cellular Genetics, The University of Texas System Cancer Center, M.D. Anderson Hospital and Tumor Institute, Houston, Texas
Abstract:Antikinetochore immunofluorescence staining has been used in several studies to determine whether a second kinetochore is present, active, or both, in multicentric chromosomes. All of these studies have used tissue culture cells, and contended with the problem of obtaining well spread, banded metaphase chromosomes without affecting the kinetochore staining. We have adapted hypotonic, centrifugation and chromosome staining techniqnes to obtain simultaneous Q-banding and bright kinetochore staining of chromosomes from human lymphocytes.
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