Silver staining of collagen fibrils in cartilage |
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Authors: | W S Hwang K Ngo and K Saito |
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Institution: | (1) Department of Pathology, University of Calgary and Foothills Hospital, 1403-29 Street N.W., T2N 2T9 Calgary, Alberta, Canada |
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Abstract: | Summary Direct visualization of individual collagen fibrils by light microscopy in human cartilage was achieved by applying a periodic acid-silver methenamine stain on plastic sections. Collagen fibrils, 100 nm in diameter or thicker, were delineated individually by light microscopy and were easily traced for a length beyond 100 m. Thinner fibrils not readily visible optically were identified if arranged in compact bundles as occurring in the superficial zone of articular cartilage. |
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