Expression of apoptosis-related genes Fas/FasL,Bax/Bcl-2 and Caspase-3 in rat corpus luteum during luteal regression |
| |
Authors: | Xinlei?Chen Hongjuan?Gao Peng?Wei Zhihong?Zhang Email author" target="_blank">Yixun?LiuEmail author |
| |
Institution: | State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China |
| |
Abstract: | Using immunohistochemistry, in situ hybridization, Western blot and TUNEL methods, we have studied the expression of Fas/FasL, Bcl-2/Bax and caspase-3 in the
corpora lutea (CL) at various stages of pseudopregnant rat induced by injection of PMSF/hCG. The results showed that no apoptotic
signal could be observed until Day 14 after hCG injection. Fas weakly expressed in the CL at all the stages increased when
luteolysis took place. FasL signal increased dramatically on Day 14 and reached the maximum level on Day 21. The expression
of Bcl-2 and Bax was detected in a time-dependent manner. At the early stage of CL development, Bcl-2 expression was stronger,
while Bax was low. The expression of Bcl-2 and Bax in the CL was completely reversed. Caspase-3 antigen could be detected
throughout all the phases of CL development in a time-dependent fashion, low on Day 2 and reaching the maximum on Day 21.
These results suggest that luteal regression at the late phases may be related to cell apoptosis. |
| |
Keywords: | Fas/FasL Bax/Bcl-2 caspase-3 luteal regression |
本文献已被 万方数据 SpringerLink 等数据库收录! |
|