Modulation by the ratio S-adenosylmethionineS-adenosylhomocysteine of cyclic AMP-dependent phosphorylation of the 50 kDa protein of rat liver phospholipid methyltransferase |
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Authors: | Mayte Villalba Isabel Varela Isabel Mérida María A Pajares Alvaro Martínez del Pozo JoséM Mato |
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Institution: | 1. Metabolismo, Nutrición y Hormonas, Fundación Jiménez Diaz, Reyes Católicos 2, 28040 Madrid Spain;2. Departamento de Bioquǐmica, Facultad de Ciencias, Universidad Complutense de Madrid, 28040 Madrid Spain |
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Abstract: | The present results show that the catalytic subunit of cyclic AMP-dependent protein kinase phosphorylates the 50 kDa protein of rat liver phospholipid methyltransferase at one single site on a serine residue. Phosphorylation of this site is stimulated 2- to 3-fold by S-adenosylmethionine. S-adenosylmethionine-dependent protein phosphorylation is time- and dose-dependent and occurs at physiological concentrations. S-adenosylhomocysteine has no effect on protein phosphorylation but inhibits S-adenosylmethionine-dependent protein phosphorylation. ratios varying from 0 to 5 produce a dose-dependent stimulation of the phosphorylation of the 50 kDa protein. In conclusion, these results show, for the first time, that the ratio can modulate phosphorylation of a specific protein. |
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Keywords: | Protein phosphorylation 50 kDa protein Phospholipid methyltransferase (Rat liver) Chaps 3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate |
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