Accumulation of inositol phosphates and cyclic AMP in guinea-pig cerebral cortical preparations: Effects of norepinephrine,histamine, carbamylcholine and 2-chloroadenosine |
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Authors: | EB Hollingsworth JW Daly |
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Institution: | 1. Laboratory of Bioorganic Chemistry, Bldg. 4, Room 212, National Institute of Arthritis, Diabetes, Digestive, Bethesda, MD 20205 U.S.A.;2. Kidney Diseases, National Institutes of Health, Bethesda, MD 20205 U.S.A. |
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Abstract: | Norepinephrine and serotonin augment by about 2-fold the accumulation of cyclic 3H]AMP elicited by 2-chloroadenosine in 3H]adenine-labeled guinea-pig cerebral cortical slices. Histamine causes a 3-fold augmentation. The first two agents have no effect on cyclic AMP alone, while histamine has only a small effect alone. The augmentation of the 2-chloroadenosine response appears to be mediated by α1-adrenergic, 5HT2-serotonergic and H2-histaminergic receptors. VIP-elicited accumulations of cyclic AMP are also augmented through stimulation of α1-adrenergic, 5HT2-serotonergic and H1-histaminergic receptors. Activation of these amine receptors also increases the turnover of phosphatidylinositols in 3H]inositol-labeled guinea pig cerebral cortical slices. Norepinephrine causes a 5-fold, serotonin a 1.2-fold, and histamine a 2.5-fold increase in accumulations of 3H]inositol phosphates. 2-Chloroadenosine, vasoactive intestinal peptide, baclofen, and somatostatin have no effect on phosphatidylinositol turnover, nor do the last two agents augment accumulations of cyclic AMP elicited by 2-chloroadenosine. The data suggest a possible relationship between turnover of phosphatidylinositol and the augmentations of the cyclic AMP accumulations elicited by biogenic amines in brain slices. |
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Keywords: | Inositol phosphate cyclic AMP Amine receptor Biogenic amine Adenosine (Guinea-pig brain) |
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