Protein phosphatases in developing Dictyostelium discoideum cells |
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Authors: | R. van Driel J.C. Arents A.P. Otte R.L. Bernstein |
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Affiliation: | Laboratory of Biochemistry, University of Amsterdam, P.O. Box 20151, 1000 HD Amsterdam The Netherlands |
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Abstract: | Protein phosphatase activities in developing Dictyostelium discoideum cells were investigated. Substrates were prepared by phosphorylation of histone H2b and kemptide (Leu-Arg-Arg-Ala-Ser-Leu-Gly) using cAMP-dependent protein kinase. Two histone phosphatase activities (Mr 170 000 and 520 000) and one kemptide phosphatase activity (Mr 230 000) were found in the cytosolic cell fraction. Histone phosphatase was also present in the particulate fraction, kemptide phosphatase was not. All phosphatase activities were present throughout development. No differences in protein phosphatase activities were found in prespore and prestalk cells. A heat-stable factor which inhibits the particulate and both soluble histone phosphatase activities was isolated. |
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Keywords: | Protein phosphatase Development Hepes 4-(-2-hydroxyethyl)-1-piperazineethanesulfonic acid |
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