Construction of small binary vectors forAgrobacterium-Mediated transformation in plants |
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| Authors: | Jeong Hwa Lim Young Na Kang Young A Kim Dae Heon Kim Inhwan Hwang |
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| Institution: | (1) Department of Life Science and Center for Plant Intracellular Trafficking, Pohang University of Science and Technology (POSTECH), 790-784 Pohang, Korea;(2) Department of Molecular Biology, Gyeongsang National University, 660-701 Chinju, Korea |
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| Abstract: | Despite the popular use of theAgrobacterium-mediated method for transforming plants, only a very limited number of binary vectors are available. In addition, those binary
vectors are rather inconvenient for plasmid manipulation because of their large size. Here, we report the construction of
small binary vectors, pBmin1 and pBminGFP1, forAgrobacterium-mediated transformation. The sizes of pBmin1 and pBminGFP1 are approximately 5.0 kb and 6.5 kb, respectively. These vectors
contain one kanamycin resistance gene, with a double promoter for expression in both bacteria and plant cells. Vector pBminGFP1
has the 35S-GFP chimeric gene as a reporter. These small binary vectors would greatly facilitate plasmid manipulation and
plant transformation. |
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| Keywords: | Agrobacterium binary vector GFP transformation |
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