Cd2+、Al3+作用下蚕豆UDS与微核相关性分析及高等植物UDS技术初探

为探讨金属离子对高等植物非按期DNA合成(UnscheduledDNASythesis,简称UDS)和微核(MCN)的诱导作用、二者之间的关联性以及利用高等植物UDS技术检测环境诱变物的可行性,利用3HTdR前体掺入法研究了Cd²⁺、Al³⁺作用下蚕豆的UDS效应。结果表明,Cd²⁺、Al³⁺均能不同程度地诱导蚕豆UDS和MCN的发生;UDS量与微核率(MCNF)之间呈负相关(r<0),但相关不显着(|r|0.05),且二者间的相关程度在Cd²⁺和Al³⁺两种金属离子作用下没有显着差别(P>0.05);利用高等植物UDS技术检测环境诱变物质,在一定受检物剂量范围内是可靠的,但超过这个剂量范围,UDS技术无法检出.

Correlation analysis between UDS and MCN in Vicia faba treated with Cd2+ and Al3+ and UDS technique in higher plants

With the method of inserting3H TdRinto DNA, this paper studied the inductive effect of Cd²⁺ and Al³⁺ on unscheduled DNA synthesis (UDS) and micronucleus (MCN) in Vicia faba, the correlation between UDS and MCN, and the feasibility of using higher plant UDStest as the indicator to detect environmental mutagens. The results indicated that both Cd²⁺ and Al³⁺ could induce UDS and MCN in Vicia faba. There was a negative relationship between UDS and MCN, but it was not significant. The relationship between UDS and MCN was similar under the treatments of Cd²⁺ and Al³⁺ respectively. It was reliable using higher plant UDS test as the indicator to detect environmental mutagens within certain dosage range.