The Escherichia coli cytochrome b556 gene, cybA, is assignable as sdhC in the succinate dehydrogenase gene cluster |
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Authors: | Hiroshi Murakami Kiyoshi Kita Hiroshi Oya Yasuhiro Anraku |
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Affiliation: | Department of Biology, Faculty of Science, University of Tokyo, Hongo, Tokyo 113, Japan;Department of Parasitology, Juntendo University, School of Medicine, Hongo, Tokyo 113, Japan |
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Abstract: | Abstract The cytochrome b556 -deficient mutant Escherichia coli K12 strain TK3D11 [7] could not grow with succinate as the sole carbon source, but could grow well on dl -lactate. This finding suggested that cytochrome b556 is primarily responsible for oxidative metabolism and utilization of succinate. 24 Amino acid residues at the amino-terminal of purified cytochrome b556 were determined. This sequence coincided completely with amino acid residues 4 to 27, predicted from the DNA sequence of the sdhC gene, one of the unassigned open reading frames of the sdh gene cluster recently reported by Wood et al. [16]. Based on these and other results, we concluded that cybA , the gene for cytochrome b556 , is assignable as sdhC . |
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Keywords: | Cytochrome b556 succinate dehydrogenase respiratory chain cybA sdhC |
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