A fast-acting cytotoxin derived from Con A-activated porcine leukocytes. I. Biochemical characteristics and target cell specificity |
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| Authors: | E Russmann M D Kramer J H Wissler V Schirrmacher |
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| Institution: | 1. Departement of Pediatrics, Division of Pediatric Nephrology, University of Florida, 32610, United States of America;2. Department of Physiology and Aging, College of Medicine, University of Florida, 32610, United States of America;3. Department of Pathology, Immunology, and Laboratory Medicine, University of Florida, College of Medicine, Gainesville, FL 32610, United States of America;4. Department of Medicine, Division of Nephrology, Hypertension, and Renal Transplantation, College of Medicine, University of Florida, 32610, United States of America;1. Medical Research Center, the First Affiliated Hospital, Wenzhou Medical University, Wenzhou 325035, China;2. Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, China;3. Department of Endocrine and Metabolic Diseases, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China;4. School of Pharmaceutical Sciences, Hangzhou Medical College, Hangzhou, Zhejiang 311399, China |
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| Abstract: | We here describe a new cytotoxin which was detected in serum-free culture supernatants of mitogen stimulated porcine leukocytes. The factor was precipitated at 35-45% (NH4)2 SO4 concentration, sensitive to heat, low pH, and trypsin, thus indicating its protein nature. Column chromatography on hydrophilic or hydrophobic matrices revealed amphiphilic properties. The smallest unit which was sufficient to induce complete target cell lysis had a molecular weight of 33K. This porcine cytotoxin (PCT) could be distinguished functionally from lymphotoxin (LT) and tumor necrosis factor (TNF). It is a fast acting mediator which exerts strong cytostatic and cytotoxic anti-tumor activity using a panel of T lymphoma target cells. |
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