Identification of cytokinins associated with mitosis in synchronously cultured tobacco cells |
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| Authors: | Nishinari, Noriko Syono, Kunihiko |
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| Affiliation: | Department of Pure and Applied Sciences, College of General Education, University of Tokyo Komaba, Meguro-ku, Tokyo 153, Japan |
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| Abstract: | The amount of endogenous cytokinins in the butanol-soluble fractionincreased about 5 times during the mitosis of synchronouslydividing cultured tobacco cells (Nicotiana tabacum cv. Xanthi).The free cytokinins were identified as trans-zeatin and zeatinribonucleoside by paper and thin layer chromatography and byhigh-performance liquid chromatography combined with mass spectrometryand bioassay. The water-soluble fraction contained zeatin ribonucleotide,isopentenyladenosine monophosphate, phosphoglucosylzeatin andphosphoglucosylzeatin ribonucleoside. Changes in the cytokininsin the water-soluble and transfer RNA fractions during the cellcycle did not support the hypothesis that they might be thesource of the butanol-soluble cytokinins which increased duringmitosis. These seem to be synthesized de novo during mitosis. (Received February 1, 1980; ) |
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