Axonal Transport of the Ca2+-Dependent Protein Modulator of 3':5'-Cyclic-AMP Phosphodiesterase in the Rabbit Visual System |
| |
| Authors: | Paul F Erickson Kenneth B Seamon Blake W Moore Robert S Lasher Lee N Minier |
| |
| Institution: | Department of Psychiatry, Washington University School of Medicine, 4940 Audnbon Avenue, St. Louis, Missouri 63110;U.S.A. and Department of Anatomy, University of Colorado Medical Center, Denver, Colorado 80262, U.S.A. |
| |
| Abstract: | Water-soluble proteins were extracted from individual retinas, optic nerves, combined optic tracts and lateral geniculate bodies, and superior colliculi of rabbits at 1, 3, and 18 days after injection of 3H]leucine into the right eye. The Ca2+-dependent protein modulator of 3':5'-cyclic-AMP phosphodiesterase (calmodulin) was isolated from these samples by a two-step polyacrylamide gel electrophoresis procedure. An analysis of the radioactivity incorporated into the total soluble proteins and the calmodulin revealed that most of the calmodulin was axonally transported at a slow rate (2--4 mm/day) and represented about 0.45% of the total transported soluble protein. |
| |
| Keywords: | Axonal transport Calmodulin Rabbit visual system |
|
|
