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3- -Hydroxysterol synthesis by the liver
Authors:H Brunengraber  J R Sabine  M Boutry  J M Lowenstein
Affiliation:1. School of Pharmacy and Food Engineering & Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Wuyi University, Jiangmen 529020, China;2. School of Pharmacy & State Key Laboratory of Applied Organic Chemistry & Collaborative Innovation Center for Northwestern Chinese Medicine, Lanzhou University, Lanzhou 730000, China;3. School of Biomedical and Pharmaceutical sciences, Guangdoong University of Technology, Guangzhou 510006;1. Laboratory of Medicinal Chemistry, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco;2. Cátedra de Química General, Instituto de Química Inorgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, 4000, Tucumán, Argentina;3. Laboratory of Pharmacology and Toxicology, Faculty of Medicine and Pharmacy, University Mohammed V in Rabat, Morocco;4. Department of Chemistry, Faculty of Science and Arts, King Khalid University, Mohail, Assir, Saudi Arabia;5. X-ray Crystallography Unit, School of Physics, Universiti Sains Malaysia, USM, Penang 11800, Malaysia;6. Laboratory of Analytical Chemistry and Bromatology, Team of Formulation and Quality Control of Health Products, Faculty of Medicine and Pharmacy, Mohammed V University in Rabat, Morocco;1. Centro Enrica Grossi Paoletti, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy;2. Lipoprotein Metabolism Laboratory, Translational Vascular Medicine Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA
Abstract:Incorporation of 3H from [3H]H2O was used to measure 3-β-hydroxysterol synthesis in rat liver. The amount of 3H incorporated has been related to the amount of carbon incorporated by measuring the incorporation of [14C]glucose and correcting for the dilution in specific activity of the labeled carbon by endogenous carbon compounds. 3-β-Hydroxysterol is inhibited in starvation and by (−)-hydroxycitrate.
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