| YAG激光照射对高山被孢霉花生四烯酸产量的影响 |
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| 引用本文： | 周蓬蓬,余龙江,李为,朱敏,何贶.YAG激光照射对高山被孢霉花生四烯酸产量的影响[J].激光生物学报,2002,11(5):372-376. |
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| 作者姓名： | 周蓬蓬 余龙江 李为 朱敏 何贶 |
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| 作者单位： | 华中科技大学生命科学与技术学院,中国湖北,武汉,430074 |
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| 摘 要： | 花生四烯酸产生菌高山被孢霉的孢子在时间为8min，距离为10cm的YAG激光剂量照射下，其致死率为75％－80％，以该剂量反复进行照射及菌种筛选，得到其最高生物量28.6g/L，油脂11.04g/L，3.10g/L的突变株T105，比对照菌株的AA产量及油脂量分别提高了2．95倍和1．71倍。通过对突变株T105的形态，代谢情况以及继代稳定性进行分析，认为YAG激光诱变育种是获得茶花生四烯酸高产菌株的有效方法。
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| 关 键 词： | YAG激光照射 高山被孢霉 花生四烯酸产量 激光诱变 菌种选育 |
| 文章编号： | 1007-7146(2002)05-372-05 |
| 收稿时间： | 2002/7/15 |
| 修稿时间： | 2002年7月15日 |
The Effect of YAG Laser on Mortierella Alpina Producing Arachiclonic Acid |
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| ZHOU Peng peng,YU Long jiang,LI Wei,ZHU Min,HE Kuang.The Effect of YAG Laser on Mortierella Alpina Producing Arachiclonic Acid[J].ACTA Laser Biology Sinica,2002,11(5):372-376. |
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| Authors: | ZHOU Peng peng YU Long jiang LI Wei ZHU Min HE Kuang |
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| Abstract: | Under dose of YAG laser (wavelength 532nm) at irradiated time of 8 min and irradiated distance of 10 cm, lethality of spores of Mortierella Alpina was 70%~75%. After the irradiated dose of YAG laser and repeatedly screen, a mutation of Mortierella Alpina T 105 whose arachidonic acid (AA) concentration and lipid in biomass were 3 times and 1.7 times of the control strains, respectively, was obtained. Through observation of figuration, analysis of metabolic curve and genetic stability of T 105 , YAG laser is considered as a hopeful mutating way for achieving Mortierella Alpina containing high AA concentration. |
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| Keywords: | arachidonic acid mortierella alpina laser induced mutation |
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