| 家蚕NPV SOD基因序列和在大肠杆菌中表达 |
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| 引用本文： | 王文兵,季平,吴峻,何家禄,吴祥甫. 家蚕NPV SOD基因序列和在大肠杆菌中表达[J]. Acta biochimica et biophysica Sinica, 1999, 0(4) |
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| 作者姓名： | 王文兵 季平 吴峻 何家禄 吴祥甫 |
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| 摘 要： | 通过ＰＣＲ 克隆了家蚕核型多角体病毒（ＢｍＮＰＶ） ＳＯＤ基因， 并在大肠杆菌中进行表达， 证明了Ｂｍ ＮＰＶＳＯＤ基因产物确有ＳＯＤ活性， 其活力单位约为５７６ ｕ／ｍＬ 培养液。ＤＮＡ 测序结果表明Ｂｍ ＮＰＶ ＳＯＤ 基因编码１５１ 个氨基酸， 与人的ＳＯＤ１ 基因的核苷酸同源性为５６ ％ ， 与ＡｃＮＰＶ 拟为的ＳＯＤ基因同源性为９７ ．２％ 。
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| 关 键 词： | 家蚕 杆状病毒 超氧化物歧化酶 表达 序列分析 |
Sequence Analysis of SOD Gene of Bombyx mori Nuclear Polyhedrosis Virus and It's Expression in E. coli |
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| WANG Wen Bing ,,JI Ping ,WU Jun ,HE Jia Lu and WU Xiang Fu. Sequence Analysis of SOD Gene of Bombyx mori Nuclear Polyhedrosis Virus and It's Expression in E. coli[J]. Acta biochimica et biophysica Sinica, 1999, 0(4) |
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| Authors: | WANG Wen Bing JI Ping WU Jun HE Jia Lu WU Xiang Fu |
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| Affiliation: | WANG Wen Bing 1,2,JI Ping 2,WU Jun 1,HE Jia Lu 2 and WU Xiang Fu 1* |
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| Abstract: | Superoxide dismutases scavenge superoxide radicals and protect cells from oxidative stress. SOD gene of Bombyx mori nuclear polyhedrosis virus has been cloned by PCR, and is expressed in E. coli with the activity of SOD being 576.13 u/mL. DNA sequence analysis shows that SOD gene of BmNPV encodes 151 amino acids. The homology between BmNPV and AcNPV of the nucleotide sequences of SOD gene is 97.2%, and 56% between BmNPV and human SOD 1. |
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| Keywords: | Superoxide dismutase BmNPV sequence analysis expression |
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