Selection of Gongronella butleri strains for enhanced chitosan yield with UV mutagenesis |
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| Authors: | Maw Theingi Tan Teck Koon Khor Eugene Wong Sek Man |
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| Institution: | Department of Biological Sciences, National University of Singapore, Lower Kent Ridge Road, 117543, Singapore, Singapore |
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| Abstract: | This paper describes the selection of Gongronella butleri strains producing higher chitosan yield using UV mutagenesis. We have devised an enzyme-linked immunosorbent assay for the selection of high chitin deacetylase (CDA) yielding strains. Mutant strains M+1, M+2 and M+7 could produce twice the extractable chitosan yield and double the CDA activity, as compared to the wild type strain. |
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