Characterization of the Photosynthetic Apparatus and Proteome of Roseobacter denitrificans |
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Authors: | Kai Tang Rui Zong Fan Zhang Na Xiao Nianzhi Jiao |
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Institution: | (1) National Key Laboratory of Marine Environmental Sciences, Xiamen University, Xiamen, 361005, China; |
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Abstract: | The phototrophic capacity of aerobic anoxygenic phototrophic bacteria endows them with a selective advantage over other heterotrophic
bacteria in the oligotrophic ocean. Here, we reported the phototrophic features and proteome of an aerobic phototrophic bacterium
Roseobacter denitrificans under starvation stress. The fluorescence induction and relaxation measurements suggested that the photosynthetic capacity
in R. denitrificans was preserved but was lower than in the photoautotrophic bacterium Rhodobacter
sphaeroides. The existence of light-harvesting complexes (LH1 and LH2) and the reaction center (RC) in the native membrane were demonstrated
through atomic force microscopy image analysis as direct evidence of their phototrophy. The homology-based LH1–RC complex
structure was proposed in which RC was the Rb. sphaeroides homolog structure surrounded by the LH1. Moreover, the protein expression profiles of cells in the stationary phase under
heterotrophic and mixotrophic conditions show that light enhanced or activated some proteins such as carbon monoxide dehydrogenase
and NifU to cope with the low levels of amino acids and carbon sources under starvation conditions. |
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