Modification of an automated liquid-handling system for reagent-jet, nanoliter-level dispensing |
| |
Authors: | Hicks J S Harker B W Beattie K L Doktycz M J |
| |
Affiliation: | Oak Ridge National Laboratory, Oak Ridge, TN, USA. |
| |
Abstract: | Reducing the scale of biochemical reactions is becoming commonplace. Examples include the screening of large libraries of chemical compounds or gene sequences. These applications demand the ability to transfer sub-microliter volumes of fluid. To this end, we have modified a Hamilton MICROLAB 2200 with high-speed solenoids and a liquid pressurization system to modulate volume delivery down to the nanoliter level. Additional modifications include the use of sapphire-tipped dispensing nozzles and a high-resolution substage to assist in the construction of DNA microarrays. Techniques for characterizing the dispensed volume are presented. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|