Proteomic analysis of prolactinoma cells by immuno-laser capture microdissection combined with online two-dimensional nano-scale liquid chromatography/mass spectrometry |
| |
| Authors: | Yingchao Liu Jinsong Wu Guoquan Yan Ruiping Hou Dongxiao Zhuang Luping Chen Qi Pang Jianhong Zhu |
| |
| Affiliation: | (1) Department of Neurosurgery, Shandong Provincial hospital affiliated to Shandong University, 250021 Jinan, China;(2) Shanghai Neurosurgical Center, Department of Neurosurgery, Huashan Hospital, Shanghai Medical College, Fudan University, 200040 Shanghai, China;(3) Department of Chemistry, Fudan University, Institutes for Biomedical Sciences, Fudan University, 200433 Shanghai, China;(4) Department of Gastroenterology, Shandong Provincial Qianfoshan Hospital affiliated to Shandong University, 250014 Jinan, China;(5) National Key Lab for Medical Neurobiology, Institutes of Brain Sciences, Fudan University, 200032 Shanghai, China |
| |
| Abstract: | Background Pituitary adenomas, the third most common intracranial tumor, comprise nearly 16.7% of intracranial neoplasm and 25%-44% of pituitary adenomas are prolactinomas. Prolactinoma represents a complex heterogeneous mixture of cells including prolactin (PRL), endothelial cells, fibroblasts, and other stromal cells, making it difficult to dissect the molecular and cellular mechanisms of prolactin cells in pituitary tumorigenesis through high-throughout-omics analysis. Our newly developed immuno-laser capture microdissection (LCM) method would permit rapid and reliable procurement of prolactin cells from this heterogeneous tissue. Thus, prolactin cell specific molecular events involved in pituitary tumorigenesis and cell signaling can be approached by proteomic analysis. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
