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The effects of various antioxidants on the development of parthenogenetic porcine embryos |
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| Authors: | Hyung Soo Yuh Dong Hun Yu Mi Jung Shin Hei Jung Kim Ki Beom Bae Dong Seok Lee Hwi Cheul Lee Won Kyong Chang Soo Bong Park Sang Gyu Lee Hum Dai Park Ji Hong Ha Byung Hwa Hyun Zae Young Ryoo |
| Affiliation: | 1. School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University, 1370 Sankyuk-dong, Buk-ku, Daegu, 702-701, Republic of Korea 2. Animal Biotechnology Division, National Institute of Animal Science, 564 Omockchen-dong, Suwon, 441-706, Republic of Korea 3. Department of Biotechnology, School of Engineering, Daegu University, 15 Jillyang, Gyeongsang, Gyeongbuk, 712-714, Republic of Korea 4. Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology, 111 Gwahangro, Yuseong-gu, Daejeon, 305-806, Republic of Korea |
| Abstract: | The major objective of this study was to improve the development rate of parthenogenetic porcine embryos. In this study, the anti-oxidative and anti-apoptotic effects of three antioxidants, β-mercaptoethanol (β-ME), α-tocopherol, and extracellular superoxide dismutase (EC-SOD), were examined on the development of parthenogenetic porcine embryos. The development rate of parthenogenetic porcine embryos to the blastocyst stage was 8.1% for control; 19.1%, 14.6%, and 5.0% for 1, 3, and 5 μM β-ME; 17.2% and 17.5% for 50 and 100 μM α-tocopherol and 12.0% and 4.0% for EC-SOD transgenic mouse embryonic fibroblast (Tg-MEF) and EC-SOD non-transgenic mouse embryonic fibroblast (NTg-MEF) conditioned medium at day 3, respectively. Here, β-ME, α-tocopherol, and EC-SOD Tg-MEF conditioned medium increased the development rate of parthenogenetic porcine embryos to the blastocyst stage (P?P?P? |
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