Supplementary factors required for serum-free culture of rat kidney cells of line NRK-49F |
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Authors: | Gary L Bradshaw George R Dubes |
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Institution: | (1) Department of Medical Microbiology, University of Nebraska College of Medicine, 42nd and Dewey Avenue, 68015 Omaha, Nebraska;(2) Department of Microbiology, Rutgers Medical School, University of Medicine and Dentistry of New Jersey, P.O. Box. 101, 08854 Piscataway, New Jersey |
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Abstract: | Summary Factors requred as supplements to basal tissue culture medium for the multiplication of cells of the cloned rat fibroblast
line called normal rat kidney 49F (NRK-49F) were identified as epidermal growth factor, fibronectin, insulin, and retinoic
acid. The requirement for fibronectin was manifested on a clean glass surface but not on the polystyrene plastic surface tested.
This set of required factors differs substantially from the factor sets required by the Madin-Darby, canine kidney (MDCK)
and LLC-PK1 pig kidney lines of epithelial cells and the baby hamster kidney 21 (BHK-21) line of fibroblasts. The serum-free medium supplemented
with the four factors supported rapid growth of NRK-49F cells when the initial cell population density was about 8,000 cells/cm2 or greater. At lower initial densities, cell multiplication was markedly increased by adding serum-free medium that had been
conditioned by NRK-49F cells. Cell growth rate in the defined serum-free medium stayed high through two serial passages but
declined in the third serial passage unless the cell-conditioned medium was added. |
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Keywords: | epidermal growth factor fibronectin growth factors for kidney cells insulin NRK-49F cells retinoic acid |
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