结扎冠状动脉后快速起搏方法建立急性心功能不全动物模型

目的探讨建立急性心功能不全动物模型的可行性。方法完全结扎犬前降支,进行快速右室起搏,使心输出量(CCO)较基础状态稳定地下降50%,分别测定基础及心输出量下降状态下的血压(AP)、血氧(SaO2)、平均右房压(mRAP)、平均肺毛压(mPCWP)、系统血管阻力(SVR)、心腔大小、左室射血分数(LVEF)、血浆肾素活性(PRA)、内皮素(ET)、尿量(UO)、血肌酐(Scr)、肌酐清除率(Ccr)。结果结扎LAD和快速右室起搏后,CCO较基础状态均稳定地下降50%,CCO降低后,AP、SaO2显著下降,mRAP、mPCWP、SVR显著升高;心脏各腔室明显扩大,LVEF显著降低;PRA、ET、Scr明显升高,UO、Ccr明显下降。结论结扎冠状动脉前降支及快速右心室起搏可成功制作急性心功能不全的动物模型。

Anmial Model of Acute Cardiac Insufficiency Induced by Coronary Artery Occulsion and Tachycardiac Stress

Objective To establish a canine model of acute cardiac insufficiency (ACI) induced by coronary artery occulsion and tachycardiac stress.Methods Studies were performed in 12 dogs using rapid ventricular pacing after the left anterior decending(LAD) coronary artery was ligated.Cardiac output(CCO) reduction was expected to 50% of the baseline.Hemodynamic measurements were performed in the dogs under different CCO conditions.Echocardiography was used to examine the cardiac size and left ventricular ejection fraction(LVEF).Blood sample was taken for examination of plasma renin (PRA) and endothelin(ET) activity.Urine output(UO),serum creatinine(Scr),creatinine clearance(Ccr)were also assayed.Results CCO was decreased by 50% steadily after right ventricular pacing following ligation of LAD.Arterial pressure and arterial oxygen saturation decreased while right atrial pressure,pulmonary capillary wedge pressure,systemic vascular resistance increased significantly.The cardiac cavity became larger while LVEF decreased.PRA,ET,Ccr increased while UO and Ccr decreased.Conclusion A canine model of ACI can successfully be created by right ventricular pacing following ligation of LAD.