Partial differentiation of rat egg cylinders in serum-free and protein-free medium |
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Authors: | Nikola kreb Floriana Buli |
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Affiliation: | Institute of Biology, Faculty of Medicine, Zagreb, Yugoslavia;Gaziosmanpasa University, Faculty of Education, Department of Computer Education and Instructional Technology, Turkey;Department of Statistics, Brigham Young University, United States;William Paterson University, Wayne, NJ, USA |
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Abstract: | Modified organ cultures of rat egg cylinders were grown for 2 weeks in Eagle's MEM without serum or with serum added at different times. Explant survival was decreased only in cultures grown for the entire 2 weeks in serum-free medium, whereas the explant growth was impeded in all but the cultures grown for 2 weeks in 50% MEM plus 50% serum. Differentiation of epidermis and cartilage in the cultures deprived of serum for the entire 2-week period was comparable to that in fully serum-supplemented medium, whereas other differentiated tissues were rare or absent. In explants cultivated without serum for only the first week, neuroblasts were scarce. |
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