Synthesis of cell-permeable peptide nucleic acids and characterization of their hybridization and uptake properties |
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| Authors: | Zhou Peng Dragulescu-Andrasi Anca Bhattacharya Birendra O'Keefe Heather Vatta Paolo Hyldig-Nielsen Jens J Ly Danith H |
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| Institution: | Department of Chemistry, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, PA 15213, USA. |
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| Abstract: | Guanidine-based peptide nucleic acid (GPNA) monomers and oligomers containing all four natural (adenine (A), cytosine (C), guanine (G), and thymine (T)) and two unnatural (2-thiouracil (sU) and 2,6-diaminopurine (D)) nucleobases have been synthesized. Thermal denaturation study showed that GPNA oligomers containing alternate D-backbone configuration bind sequence-specifically to DNA and, when incubated with mammalian cells, localized specifically to the endoplasmic reticulum (ER). |
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