Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells |
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| Authors: | Owen Dylan M Lanigan Peter M P Dunsby Christopher Munro Ian Grant David Neil Mark A A French Paul M W Magee Anthony I |
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| Institution: | Chemical Biology Centre, Department of Physics, Imperial College London, London, United Kingdom. dylan.owen@imperial.ac.uk |
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| Abstract: | We apply fluorescence lifetime imaging to the membrane phase-sensing dye di-4-ANEPPDHQ in model membranes and live cells. We show that the 1700 ps lifetime shift between liquid-disordered and liquid-ordered phases offers greater contrast than the 60 nm spectral shift previously reported. Detection of cholesterol-rich membrane microdomains is confirmed by observation of the temperature dependence of membrane order and by cholesterol depletion using methyl-beta-cyclodextrin. |
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