Biochemical and biophysical analysis of plasmid pMJ600-encoded tellurite [TeO32−] resistance |
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Authors: | G. Lloyd-Jones D.A. Ritchie P. Strike |
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Affiliation: | Laboratory of General Microbiology, University of Geneva, Switzerland. |
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Abstract: | The actin of Neurospora crassa wild type Strain St. Lawrence has been purified, characterized and localized. A fungal 43 kDa protein was isolated by affinity chromatography on DNase I-Sepharose. This protein was identified as actin on immunoblots when an anti-actin monoclonal antibody raised against chicken gizzards was used as a probe. After two-dimensional gel electrophoresis three actin isoforms were detected. The distribution of actin in hyphae was examined by FITC-phalloidin staining of formaldehyde fixed hyphae. F-actin was found to be mainly concentrated in the hyphal tips in which it formed a uniform cap. Apical actin could be involved in hyphal morphogenesis, organelle motility and maintenance of polarity. |
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Keywords: | Helicobacter pylori Urease Spiral gastric bacteria |
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