Ionic strength effect on adsorption of cellobiohydrolases I and II on microcrystalline cellulose |
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| Authors: | DW Kim YG Hong |
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| Institution: | (1) Department of Chemistry, College of Natural Sciences, Chungbuk National University, Cheongju, 361-763, Korea |
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| Abstract: | Cellobiohydrolase I (CBH I) has a higher adsorption affinity (K
ad) and tightness (– H
a) for Avicel than cellobiohydrolase II (CBH II). The adsorption processes of CBH I and II were exothermic, and the degree of exothermy were larger with the increasing ionic strength. Entropy change of CBH I was larger than CBH II with increasing ionic strength. CBH I was more effective than CBH II for binding at a given ionic strength. |
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| Keywords: | adsorption cellulase cellulose ionic strength thermodynamics |
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