The carbohydrate constituents of the cell wall of suspension cultures of Rosa glauca

Sequential extractions of 14-day-old Rosa glauca cell walls cultured in vitro showed that two different types of acidic polysaccharide were present. One was extracted with EDTA or ammonium oxalate solutions, and the other remained in close association with cellulose even after 4.3 N NaOH extractions or 2 N H₂SO₄ hydrolysis. The cell wall has a low content in structural protein. The behaviour of each constituent sugar was followed during the course of the various extraction steps, and a complete quantitative account of the protein, uronic acid and neutral sugar components is given at each stage.