Radioimmunoassay for the quantitative determination of scopolamine |
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Authors: | Elmar W Weiler Joachim Stöckigt Meinhart H Zenk |
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Institution: | Lehrstuhl für Pflanzenphysiologie, Ruhr-Universität Bochum, D 4630 Bochum, West Germany |
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Abstract: | A radioimmunoassay for the determination of pmol amounts of the tropane alkaloid scopolamine has been developed. The assay uses tritiated N-C3H3]scopolamine of high specific activity (0.67 Ci/mmol) as tracer. The measuring range of the assay extends from 0.5 to 50 ng of scopolamine, and as little as 200 pg may be detected. The antiserum raised against a conjugate of scopolamine-N-β-propionic acid-human serum albumin is highly specific, and neither hyoscyamine, 6-hydroxyhyoscyamine, scopine, tropic acid nor other related alkaloids interfere in the scopolamine determination in crude plant extracts. This assay allows for the first time the rapid, sensitive and precise (CV = 2.5 %) determination of this alkaloid in unpurified extracts of scopolamine-containing plants. The distribution of scopolamine in Datura plants, as well as its diurnal changes in leaf concentrations, has been investigated in detail and a preliminary survey on the variability of scopolamine leaf concentrations in a population of Datura sanguinea plants is given. |
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Keywords: | Solanaceae radioimmunoassay scopolamine |
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