Fractionation of globulins of milled rice |
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Authors: | Cynthia G Pascual Bienvenido O Juliano Yonemi Tanaka |
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Institution: | Chemistry Department, The International Rice Research Institute, Los Baños, Laguna, Philippines |
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Abstract: | Globulins were prepared by repeated precipitation with 1.3 M (NH4)2SO4 from a 0.7 M NaCl extract of milled rice. Isoelectric precipitation at pH 4.5 did not effectively remove the α-globulin from the others. A major fraction that remained in solution during dialysis of the globulin precipitate against water was similar in properties to the globulin soluble at pH 4.5 during the isoelectric precipitation process. Some properties of this water-soluble globulin fraction are reported. Proteins extracted from milled rice at 50° with 0.5 M NaCl and precipitated as 1- to 3-μm particles on cooling were verified to be globulins. |
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Keywords: | Gramineae milled rice globulins isoelectric focusing |
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