Identification of multiple forms of phosphofructokinase in ripening dwarf cavendish banana |
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Authors: | PM Nair BG Darak |
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Institution: | Biochemistry and Food Technology Division, Bhabha Atomic Research Centre, Bombay 400085, India |
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Abstract: | The levels of six glycolytic intermediates and the activity of phosphofructokinase (PFK) were determined in Dwarf Cavendish banana at different stages of ripening between harvest and senescence. There was a 2.3-fold increase in the level of fructose- 1,6-diphosphate between the preclimacteric and climacteric peak stage. The PFK preparations from preclimacteric and climacteric peak stages were purified ca 15-fold using Blue-Sepharose affinity chromatography. The clectrophoretic studies with the enzyme preparations ofthese two stages ofripening indicated the presence of two forms of PFK at both stages of ripening. |
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Keywords: | Musaceae banana fruit ripening phosphofructokinase multipleforms: control of climacteric |
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