An arabinogalactoxyloglucan from the cell wall of Solanum tuberosum |
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Authors: | Stephen G. Ring Robert R. Selvendran |
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Affiliation: | Agricultural Research Council, Food Research Institute, Colney Lane, Norwich NR4 7UA, U.K. |
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Abstract: | Cell-wall material from potatoes was fractionated by successive extractions with water at 80°, 0.2 M (NH4)2C2O4 at 80°, 1 M and 4 M KOH, to leave a residue of α-cellulose. The compositions of the isolated carbohydrate polymers were determined by sugar and methylation analysis. From the 4M KOH-soluble fraction an arabinogalactoxyloglucan was isolated and (partially) characterized by methylation analysis of the undegraded polymer and partially degraded methylated polymer. Methylation analysis of the oligosaccharides produced on treatment of the xyloglucan with cellulase threw additional light on the structural features of the polysaccharide. The results show that the xyloglucan has a cellulosic backbone which is highly substituted at position 6 with xylopyranose residues, some of which, in turn, carry either arabinofuranose or galactopyranose residues, as a substituent on position 2. The significance of these results is discussed. |
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Keywords: | Solanaceae potatoes cell-wall material arabinogalactoxyloglucan cellulase degradation oligosaccharides methylation analysis. |
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