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Long-term stability in biomass and production of terpene indole alkaloids by hairy root culture of Rauvolfia serpentina and cost approximation to endorse commercial realism |
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| Authors: | Pallavi Pandey Ranjeet Kaur Sailendra Singh Sunil Kumar Chattopadhyay Santosh Kumar Srivastava Suchitra Banerjee |
| Institution: | 1. Plant Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India 2. Process Chemistry & Chemical Engineering Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India 3. Medicinal Chemistry Division, Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, Uttar Pradesh, India |
| Abstract: | The effect of 6 years of cultivation and use of table-sugar (TS) on the biomass/terpene alkaloid productivities and rol gene expression were studied in a hairy root (HR) clone of Rauvolfia serpentina. The media cost could be reduced >94 % by replacing sucrose (SUC) with TS—an unexplored avenue for HR cultivation. The overall productivities increased over long-term cultivation with sugar proving superior to SUC for biomass (24.4 ± 2.11 g/l DW after 40 days to 17.31 % higher) and reserpine (0.094 ± 0.008 % DW after 60 days to 193.8 % more) production. The latter however revealed comparatively better yields concerning ajmaline (0.507 ± 0.048 % DW after 60 days to 61.98 % higher) and yohimbine (0.628 ± 0.062 % DW after 60 days to 38.32 % higher), respectively. PCR amplification of rol genes confirmed long-term expression stability. |
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