KfoE encodes a fructosyltransferase involved in capsular polysaccharide biosynthesis in Escherichia coli K4 |
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Authors: | Jia Liu Aihua Yang Jie Liu Xiaofan Ding Liming Liu Zhongping Shi |
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Affiliation: | 1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China 2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, Jiangsu, China 3. Jiangsu Jiangshan Pharmaceutical Co., Ltd., Jingjiang, 214500, China
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Abstract: | Escherichia coli K4 synthesizes a capsular polysaccharide (CPS) consisting of a fructose-branched chondroitin (GalNAc-GlcA(fructose)n), which is a biosynthetic precursor of chondroitin sulfate. Here, the role of kfoE in the modification of the chondroitin backbone was investigated using knock-out and recombinant complementation experiments. kfoE disruption and complementation had no significant effect on cell growth. CPS production was increased by 15 % in the knock-out strain, and decreased by 21 % in the knock-out strain complemented with recombinant kfoE. CPS extracted from the knock-out strain was chondroitin, whereas CPS extracted from the complemented strain was a fructose-branched chondroitin. The results demonstrated that the kfoE gene product altered the fructose group at the C3 position of the GlcA residue during production of K4CPS. |
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