Stimulatory effects of amino acids and oleic acid on poly(3-hydroxybutyric acid) synthesis by recombinant Escherichia coli |
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| Affiliation: | 1. Department of Chemical Engineering, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea;2. BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea;1. School of Environmental Sciences, Jawaharlal Nehru University, New Delhi 110 067, India;2. Amity School of Earth and Environmental Sciences, Amity University Haryana, Manesar, Gurugram 122413, India;1. Structural and Functional Biochemistry, Laboratory of Proteomics and Metabolic Engineering of Prokaryotes, Department of Life Sciences and Systems Biology, University of Torino, Via Accademia Albertina 13, 10123, Torino, Italy;2. Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA;1. Department of Materials Science and Metallurgical Engineering, Electronic Microscopy Laboratory, Polytechnic University of Catalonia. Eduard Maristany 10-14, 08930, Barcelona, Spain;2. Department of Materials Science and Metallurgical Engineering, Biomaterials, Biomechanics and Tissue Engineering Group, Polytechnic University of Catalonia. Eduard Maristany 10-14, 08930, Barcelona, Spain;3. CIBER-BBN, Centro de Investigación Biomédica en Red, Madrid, Spain;1. Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA;2. Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA;3. Bioenergy Science Center, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA;4. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA;1. Ecobiomaterial Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Penang, Malaysia;2. Centre for Chemical Biology, Universiti Sains Malaysia, 10 Persiaran Bukit Jambul, Bayan Lepas, 11900 Penang, Malaysia;1. Department of Biological Sciences, Dartmouth College, Hanover, NH 03755, USA;2. Center for Bioenergy Innovation, Oak Ridge National Laboratory, Oak Ridge, TN 37830, USA;3. Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA |
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| Abstract: | Addition of cysteine, isoleucine, methionine, or proline promoted poly(3-hydroxybutyric acid) [PHB] synthesis by recombinant Escherichia coli more than two-fold. Oleic acid also enhanced PHB synthesis more than three-fold. A PHB concentration of 70 g/l could be obtained by fed-batch culture of recombinant E. coli in a defined medium supplemented with small amounts of isoleucine, methionine, and proline. The stimulatory effects of amino acids and oleic acid on PHB synthesis seems to be due to the availability of more acetyl-CoA and/or NADPH. |
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